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HPLC CHROMATOGRAMS | CLINICAL/FORENSIC
Pain Management Drugs Pain Management Drugs in Urine on Ultra II® Biphenyl (LC/MS/MS)
NEW! Peaks RT (min.) 1. Acetaminophen 1.18 2. Morphine 1.79 3. Codeine 3.21 4. Oxycodone 3.46 5. Hydrocodone 3.60 6. Fentanyl 6.61 7. Buprenorphine 6.66 8. Lorazepam 6.79 9. Diazepam 8.06 10. Methadone 8.21
MRM1 152/110 286/157 300/165 316/298 300/199 337/132 468/187 321/275 285/154 310/77
MRM2 152/65 286/181 300/215 316/241 300/171 337/216 468/84 321/229 285/193 310/223
Recommended for opiates.
LC_CF0516
Column Dimensions: Particle Size: Pore Size: Temp.: Sample Conc.: Inj. Vol.: Mobile Phase
Detector Model #: Ion Source: Ion Mode: Curtain Gas: Gas 1: Gas 2: Source Temp.: Source Voltage: Mode: Dwell Time: Instrument
Ultra II® Biphenyl (cat.# 9609352) 50 mm x 2.1 mm ID 3 µm 100 Å 40 °C 5 ng/mL in 1:10 urine:mobile phase 5 µL A: 0.1% formic acid in water B: 0.1% formic acid in methanol Time (min.) Flow (mL/min.) 0 0.5 10 0.5 10.1 0.5 12 0.5
%A 90 0 90 90
%B 10 100 10 10
Acknowledgement
Applied Biosystems/MDS Sciex LC/MS/MS API-5000™ TurboIonSpray® ESI+ 25 psi (172.4 kPa) 60 psi (413.7 kPa) 40 psi (275.8 kPa) 550 °C 2000 V MRM 50 ms Applied Biosystems/MDS Sciex LC/MS/MS System Special thanks to Applied Biosystems for providing instrument time.
For more information on Biphenyl columns, visit
www.restek/biphenyl www.restek.com Website : www.chromtech.net.au E-mail :
[email protected] TelNo : 03 9762 2034 . . . in AUSTRALIA
747 Mar 2011
