Rtx09 527

510-533_HPLCapps.qxp 11/19/2008 3:16 PM Page 527 HPLC APPLICATIONS | PHARMACEUTICAL Antibiotics, Antidepressants An...

0 downloads 149 Views 136KB Size
510-533_HPLCapps.qxp

11/19/2008

3:16 PM

Page 527

HPLC APPLICATIONS | PHARMACEUTICAL

Antibiotics, Antidepressants Antibiotic Cephaloridine on Ultra IBD (Reversed Phase)

Get More!

1

Peak List: 1. cephaloridine Sample: Inj.: Conc.: Solvent:

5µL 1mg/mL acetonitrile:water (50:50, v/v)

Column: Cat.#: Dimensions: Particle size: Pore size:

acetonitrile: 20mM ammonium phosphate, pH 4.0 (20:80, v/v) 1.2mL/min. 27°C UV @ 254nm

Flow: Temp.: Det.:

1

2

3

4

5

www.restek.com/pharmaceutical

Ultra IBD 9175565 150mm x 4.6mm 5µm 100Å

Conditions: Mobile phase:

0

Pharmaceutical Related Articles Online

6

7

8

min.

LC_0102

Amitriptyline on Ultra IBD, pH 7

Antidepressants (Tricyclic) on Allure® Basix Peak List: 1. doxepin 2. nordoxepin 3. amitriptyline 4. imipramine 5. desimipramine 6. nortriptyline 7. protriptyline

Peak List 1. amitriptyline Sample: Inj.: 10µL Conc.: ~100µg/mL Sample diluent: mobile phase Column: Cat.#: Dimensions: Particle size: Pore size: Conditions: Mobile phase: Flow: Temp.: Det.:

10µL 100µg/mL each mobile phase

Column: Cat.#: Dimensions: Particle size: Pore size:

Allure® Basix 9161565 150mm x 4.6mm 5µm 60Å

Conditions: Mobile phase: acetonitrile: 50mM KH2PO4 pH3:methanol (60:25:15, v/v/v) Flow rate: 1.2mL/min. Temp.: 30°C Det.: UV @ 254nm

1 2

Ultra IBD 9175565 150mm x 4.6mm 5µm 100Å

Sample: Inj.: Conc.: Solvent:

3

20mM potassium phosphate (pH 7):methanol, 10:90 1.0mL/min. ambient UV @ 254nm

4 6 5

7

LC_PH0444

0

1

2

3

4

5

6

7

8

9 min.

LC_0053

800-356-1688 or 814-353-1300

www.restek.com

527