510-533_HPLCapps.qxp
11/19/2008
3:16 PM
Page 527
HPLC APPLICATIONS | PHARMACEUTICAL
Antibiotics, Antidepressants Antibiotic Cephaloridine on Ultra IBD (Reversed Phase)
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Peak List: 1. cephaloridine Sample: Inj.: Conc.: Solvent:
5µL 1mg/mL acetonitrile:water (50:50, v/v)
Column: Cat.#: Dimensions: Particle size: Pore size:
acetonitrile: 20mM ammonium phosphate, pH 4.0 (20:80, v/v) 1.2mL/min. 27°C UV @ 254nm
Flow: Temp.: Det.:
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www.restek.com/pharmaceutical
Ultra IBD 9175565 150mm x 4.6mm 5µm 100Å
Conditions: Mobile phase:
0
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min.
LC_0102
Amitriptyline on Ultra IBD, pH 7
Antidepressants (Tricyclic) on Allure® Basix Peak List: 1. doxepin 2. nordoxepin 3. amitriptyline 4. imipramine 5. desimipramine 6. nortriptyline 7. protriptyline
Peak List 1. amitriptyline Sample: Inj.: 10µL Conc.: ~100µg/mL Sample diluent: mobile phase Column: Cat.#: Dimensions: Particle size: Pore size: Conditions: Mobile phase: Flow: Temp.: Det.:
10µL 100µg/mL each mobile phase
Column: Cat.#: Dimensions: Particle size: Pore size:
Allure® Basix 9161565 150mm x 4.6mm 5µm 60Å
Conditions: Mobile phase: acetonitrile: 50mM KH2PO4 pH3:methanol (60:25:15, v/v/v) Flow rate: 1.2mL/min. Temp.: 30°C Det.: UV @ 254nm
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Ultra IBD 9175565 150mm x 4.6mm 5µm 100Å
Sample: Inj.: Conc.: Solvent:
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20mM potassium phosphate (pH 7):methanol, 10:90 1.0mL/min. ambient UV @ 254nm
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LC_PH0444
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9 min.
LC_0053
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