Cocaine, Benzodiazepines
527
Cocaine and Ecgonine Methyl Ester on Allure® PFP Propyl
Peak List: 1. EME (ecgonine methyl ester) 2. COC (cocaine) 10µL 1µg/mL water 4°C
Column: Cat.#: Dimensions: Particle size: Pore size:
Allure® PFP Propyl 9169532 30mm x 2.1mm 5µm 60Å
Flow: Column temp.: Det.: Interface: Interface temp.: Ion mode: ESI probe voltage: Orifice: Ring: Collision gas: Collision gas pressure: Collision gas energy:
1
XIC of +MRM (3 pairs) for 200.0 / 182.0 amu
0
0.5
1.0
1.30e3 cps
1.5
2.0
2.5
3.0
3.5
4.0
4.5
min.
2
XIC of +MRM (3 pairs) for 304.0 / 182.0 amu
2.35e3 cps
0 0.5 1.0 1.5 2.0 2.5 Data Courtesy of Shane Needham, Pfizer Inc.
3.0
3.5
4.0
4.5
Electron multiplier: Auxilary gas flow: Nebulizer gas setting: Curtain gas setting:
5mM ammonium formate, pH 3.0: acetonitrile (10:90, v/v) 0.6mL/min. ambient Applied Biosystems/MDS SCIEX API 3200™ MS/MS system turbo ion spray, ESI 150°C positive 5000V + 71V + 265V nitrogen 2.2 mTorr 28 eV (COC) 26 eV (EME) 2100 volts 7000cc/min. 15lb/in.2 12lb/in.2
min.
LC_0126
Benzodiazepines on 1.9µm Pinnacle™ DB PFP Propyl
new!
B) 1.9µm Pinnacle™ DB PFP Propyl
A) 1.9µm C18
4
Peak List: Conc (µg/ml) 1. 7-amino clonazepam* 20 2. 7-amino flunitrazepam* 20 3. bromazepam 100 4. oxazepam 100 5. lorazepam 100 6. clonazepam 100 7. nitrazepam 100 8. nordiazepam 100 9. flunitrazepam 100 10. diazepam 100 * metabolite
6
Compounds coelute on a C18 column
9
7
10
5 8
Sample: Inj.: 1µL Conc.: as listed Sample diluent: starting mobile phase (80:20 A:B) Column:
LC_PH0451
Cat.#: Dimensions: Particle size: Pore size:
3
Condittions: Mobile phase:
2 1 Flow: Temp.: Det.:
LC_PH0452
1
2
3
4
A. 1.9µm C18 B. 1.9µm Pinnacle™ DB PFP Propyl A. 9414212 B. 9419212 100mm x 2.1mm 1.9µm 140Å A: 0.1% formic acid in water B: 0.1% formic acid in acetonitrile Time (min.) %B 0 20 1 20 6 80 0.6mL/min. 40°C UV @ 254nm
5 min.
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Sample: Inj.: Conc.: Solvent: Temp.:
Conditions: Mobile phase:
