526
Opiates, Cannabinoids new! Opiates on Allure® PFP Propyl
H P LC A P P L I C AT I O N S | F O R E N S I C
1. morphine 2. oxymorphone 3. hydromorphone 4. codeine 5. 6-monoacetylmorphine 6. oxycodone 7. hydrocodone
Sample: Inj.: Conc.: Solvent:
opiates 10µL 25ug/mL methanol
Column: Cat.#: Dimensions: Particle size: Pore size:
Allure® PFP Propyl 9169552 50mm x 2.1mm 5µm 60Å
Conditions: Mobile phase:
A: 0.1% formic acid in water B: 0.1% formic acid in 80:20, methanol:acetonitrile Time: 0.0 3.00 6.00 6.10 8.10
Flow: Temp.:
0.40mL/min. 30°C
Det.: Ion source: IonSpray voltage: Gas 1: Gas 2: Source temp.:
Applied Biosystems/MDS Sciex API 3200™ MS/MS system Electrospray, positive 5500 65psi (448kPa) 45psi (310kPa) 600°C
Compoound morphine morphine hydromorphone hydromorphone oxymorphone oxymorphone codeine codeine hydrocodone hydrocodone oxycodone oxycodone 6-monoacetylmorphine 6-monoacetylmorphine
LC_PH0454
%B 10 50 50 10 Stop pumps
Q1 286 286 286 286 302 302 300 300 300 300 316 316 328 328
Q3 152 165 185 157 227 198 152 115 199 128 240 256 211 193
Mass Spectrometer Experiments: Declustering Collision Potential (V) Energy (V) 46 79 46 51 46 41 46 55 36 37 36 55 46 85 46 89 46 39 46 69 31 39 31 33 51 55 51 35
new!
Cannabinoids by LC/MS/MS on Allure® Biphenyl (extracted ions) 1. 2. 3. 4. 5. 6.
11-hydroxy-∆9-tetrahydrocannabinol cannabidiol 11-nor-9-carboxy-∆9-tetrahydrocannabinol cannabinol ∆9-tetrahydrocannabinol ∆9-tetrahydrocannabinol-d3
Sample: Inj.: Conc.:
10µL 100ng/mL each component Sample diluent: mobile phase Column: Cat.#: Dimensions: Particle size: Pore size: Conditions: Mobile phase:
Allure® Biphenyl 9166332 30mm x 2.1mm 3µm 60Å A: 0.1% formic acid in water B: 0.1% formic acid in methanol Time (min.) 0.5 2.5 5.0 6.0 6.1
Flow: Temp.: Det.:
Interface: Ion mode: Temp.: Ion source:
LC_PH0424
Phone: 800-356-1688 or 814-353-1300 www.restek.com
%B 50 90 90 50 —
0.5mL/min. ambient Applied Biosystems/ MDS SCIEX API 3200™ MS/MS system ESI positive 500°C 5500V
