Product Specification Sheet α (spectrin α) Antibodies Fodrin-α Cat # FOD11-P
Human Fodrin-α control/blocking peptide
SIZE: 100 ug
Cat # FOD11-S
Rabbit Anti-Human Fodrin-α antiserum
SIZE: 100 ul
Cat # FOD11-A
Rabbit Anti-Human Fodrin-α IgG, aff pure
SIZE: 100 ug
Glutamate is the main excitatory neurotransmitter in the brain. To date five glutamate Transporters have been cloned: GLAST (EAAT1), GLT1 (EAAT2), EAAC1 (EAAT3), EAAT4, and EAAT5. These transporters are believed to be critical in reducing potentially toxic extracellular concentration of glutamate by rapid uptake into nerve terminals and glial cells. Glutamergic neurotransmission occurs through an exocytotic process involving the interaction of glutamate containing synaptic vesicles with the plasma membranes of the presynaptic ending. Recently a protein, termed inhibitory protein factor (IPF), has been isolated from brain that inhibits glutamate and GABA uptake into synaptic vesicles (IC50 ~25 nM). IPF does not inhibit ATP-independent uptake, norepinephrine uptake into chromaffin vesicles, and Na-dependent glutamate uptake into synaptosomes. IPF refers to a three distinct proteins with ~mol wt of 138kDa (IPF-α), 135 kDa (IPF-β), and 132 kDa (IPF-γ). IPF-α is derived from a ubiquitous, non-erythroid brain spectrin called αFodrin, a well-characterized protein previously implicated in exocytosis/endocytosis, apoptosis, and NMDA-receptor activation. However, α-Fodrin itself has no effect on glutamate uptake. The Nterminal 1-20 aa of IPF-α, IPF-β, and IPF-γ are identical with 26-45 aa of α-Fodrin (mol wt ~240 kDa). Therefore, it appears that some identified protease(s) may generate IPF-α from α-Fodrin.
Source of Antigen and Antibodies Antigen
Ab Host/type
2-Ab -ve control
Human fodrin-α is 2472 aa protein . A 10-aa peptide from fodrin-α (1); Designation (#FOD11-P,
control/blocking peptide) conjugated to KLH. Epitope location ~ N-terminus Rabbit, Polyclonal unpurified antiserum (#FOD11-S) and IgG, purified over antigen-agarose (Cat # FOD11-A) Cat # 20320, goat anti-rabbit IgGHRP (AP, biotin, FITC conjugates also available). # 20009-1, Rabbit (non-immune) IgG, purified, suitable for ELISA, Western, IHC as –ve control
Form & Storage of Antibodies/Peptide Control Antiserum (unpurified) 100ul solution lyophilized powder Supplied in Buffer: 0.05% azide Reconstitute powder in 100 ul PBS Affinity pure IgG 100 ug/100ul solution lyophilized powder Supplied in Buffer: PBS+0.1% BSA Reconstitute powder in PBS at 1mg/ml
Control/blocking peptide 100 ug/100 ul solution lyophilized powder Supplied in Buffer: PBS pH 7.5, Reconstitute powder in PBS at 1 mg/ml. Storage
Short-term: unopened, undiluted liquid vials at 20oC and powder at 4oC or -20oC.. Long-term: at –200C or below in suitable aliquots after reconstitution. Do not freeze and thaw and store working, diluted solutions. Stability: 6-12 months at –20oC or below. Shipping: 4oC for solutions and room temp for powder
Recommended Usage Western Blotting 1:1K-5K for antiserum and 1-10 ug/ml for aff pure IgG using Chemiluminescence technique. IPF’s are approx. ~240 kDa. ELISA: Control peptide can be used to coat ELISA plates at 1 ug/ml and detected with antibodies (1:10-50K for antiserum serum and 0.5-1 ug/ml for affinity pure IgG). Histochemistry & Immunofluorescence: not tested
Specificity & Cross-reactivity Human FOD11-P sequence is 100% conserved in rat, canine, and chicken fodrin-α. Antibody crossreactivity in various species is not established. Control peptide, because of its low mol. Wt (
