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pISSN, eISSN 0125-5614 M Dent J 2017; 37 (1) : 23-28 Original Article The effect of LED light on viability and prolife...

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pISSN, eISSN 0125-5614 M Dent J 2017; 37 (1) : 23-28

Original Article

The effect of LED light on viability and proliferation of periodontal ligament fibroblast cells Thamonwan Langlongsathit1, Warunyu Sermkasemsin2, Orakot Leysak3, Rudee Surarit4, Pirasut Rodanant5 1 2 3 4 5

Soidao Hospital, Chanthaburi, 22180. Phimai Hospital, Nakhon Ratchasima 30110. Surat Thani Hospital, Surat Thani 84000. Department of Oral Biology, Faculty of Dentistry, Mahidol university, Bangkok, Thailand 10400. Department of Advanced General Dentistry, Faculty of Dentistry, Mahidol university, Bangkok, Thailand 10400.

Objective: To evaluate the effect of LED blue light on the viability, proliferation and morphology of periodontal ligament fibroblasts (PDLF). Materials and methods: Human PDLF cells were cultured and irradiated with LED blue light at different intensity and duration. MTT assay was used to determine the cytotoxicity and proliferation activity. Cell morphology was observed under light microscope. Statistical analysis was performed using ANOVA at a significant level of p